Extended Data Fig. 2: RTEL1 is essential for MiDAS in telomerase positive cells under replication stress conditions.
From: RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome
a, Experimental workflow for the analysis of MiDAS in prometaphase HeLa or HCT116 cells following RTEL1 depletion and APH treatment. b & c, Representative images (b) and quantification (c) of RTEL1 expression following siRNA transfection with a control and RTEL1 siRNAs. Tubulin was used as a loading control. The quantification of relative protein levels was performed using Fiji/ImageJ software. Error bars indicate mean ± s.d. (N = 3). ****P < 0.0001, based on two-tailed Student’s t-test d & e, Representative IF images (d) and quantification (e) of MiDAS (indicated with EdU; red) in prometaphase cells. DNA was stained with DAPI (blue). Error bars represent median ± s.e.m. (N = 3). ****p < 0.0001, based on two-tailed non-parametric Mann Whitney test. Scale bar is 10 µm. Data for graphs in panels c,e are available as Source data. Uncropped image for panel b is available as Source data.
