Extended Data Fig. 5: RTEL1 depletion induces chromosome fragility at common fragile sites.
From: RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome
a, Experimental workflow for analyzing chromosome fragility in U2OS cells. b, Representative images of FISH signal on chromosomes from cells treated with either an siRNA control (siCon), an siRNA targeting RTEL1 (siRTEL1), or 0.4 μM APH. n, the number of loci examined. Loci of FRA16D (green) or FRA3B (red) were detected by FISH probes. DNA was stained with DAPI. c, Quantification of chromosome fragility at FRA16D or FRA3B. Error bars indicate mean ± s.d. (N = 3). *p < 0.05; **p < 0.01, based on two-tailed Student’s t-test. Scale bar is 2 µm. Data for graphs in panel c is available as Source data.
