Extended Data Fig. 8: SETX does not play a role in MiDAS.
From: RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome
a, Experimental workflow for analyzing prometaphase U2OS cells by IF following SETX depletion and APH treatment. b, Representative western blotting analysis of SETX following siRNA treatment. Actin was used as a loading control. c, d, Representative images of MiDAS (labeled with EdU; red) (c) and quantification (d) in prometaphase U2OS cells following SETX depletion. DNA was stained with DAPI (blue). Error bars represent median ± s.e.m. (N = 3). ns: p > 0.05, based on two-tailed non-parametric Mann Whitney test. Data for graphs in panel d is available as Source data. Uncropped image for panel b is available as Source data.
