Extended Data Fig. 8: Transcription is toxic to replication in absence of SLX4-RTEL1 complex formation.
From: SLX4 interacts with RTEL1 to prevent transcription-mediated DNA replication perturbations
(a) Supporting data for the DNA fiber assay shown in Fig. 6a. (b) HeLa “Fit0” cells were depleted for SLX4 or RTEL1. 1 µM triptolide was added to the culture medium for 3 h before and during the IdU and CldU pulses to inhibit transcription initiation. Replication fork dynamics was analysed as in Fig. 3a. Mann-Whitney test, ns: not significant, **: p < 0.01, ****: p < 0.0001). Uncropped images of the immunoblots and data for graphs in panels a and b are available as Source data.
