Extended Data Fig. 7: TAX-4 and lipid interactions.
From: Mechanism of ligand activation of a eukaryotic cyclic nucleotide−gated channel
a, Cryo-EM density map of cGMP-unbound TAX-4 reconstituted in lipid nanodiscs. Densities colored in blue are not modeled into TAX-4 and likely represent lipids. b,c, Possible lipid binding in several different regions in the closed state. The extra densities were fit with phosphatidylcholine (lipid 1 and 2) or phosphatidic acid (lipid 3 and 4). d, Cryo-EM density map of cGMP-bound TAX-4 reconstituted in lipid nanodiscs. Densities colored in blue are not modeled into TAX-4 and likely represent lipids. e, Possible lipid binding in the open state. The extra densities were fit with phosphatidylcholine (lipid 1 and 2) or phosphatidic acid (lipid 3). Density maps were contoured at 5σ (b,e) or 3σ (c).
