Extended Data Fig. 10: Association of Ycs4 with Smc2–Smc4 prevents Ycg1 binding. | Nature Structural & Molecular Biology

Extended Data Fig. 10: Association of Ycs4 with Smc2–Smc4 prevents Ycg1 binding.

From: Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism

Extended Data Fig. 10

a, Size exclusion chromatography of complexes formed between a trimeric Smc2–Smc4–Brn1NC and either Ycs4–Brn1Ycs4 (left), Ycg1–Brn1Ycg1 (center) or both (right). Peak fractions were analyzed by SDS-PAGE and Coomassie Blue staining. b, as in a, but in the presence of 1 mM ATP. One chromatography run of two independent repeats shown for each experiment.

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