Extended Data Fig. 4: Identification of TIS sequence features in uORF reporters enriched in ribosome fractions.
From: Decoding mRNA translatability and stability from the 5′ UTR
a, A total of 14,381 sequences with all permutations of NNNNAUGNNN are ranked based on the ratio of frequency between monosome and polysome. Both the top and bottom hits are highlighted. b, Sequence logo of 10 nt random sequences with high (top) or low (bottom) M/P ratio. Note that the high M/P sequence is consistent with the Kozak consensus sequence. c, A violin plot shows the ratio of relative frequencies of inserted sequences in monosome over polysome, when ATG triplet is followed by different nucleotides. d, Representative flow cytometry scatterplots of HEK 293-Kb cells transfected with mRNA reporters with sequence variants chosen from monosome (M1 - M4) or polysome (P1 - P4).
