Extended Data Fig. 5: uORF translation triggers mRNA decay in a ribosome-dependent manner.
From: Decoding mRNA translatability and stability from the 5′ UTR
a, A scatter plot shows the correlation of triplet frequencies enriched in polysome and GFPH population from HEK293-Kb cells transfected with mRNA reporters. All points are color-encoded based on the similarity to ATG. b, A scatter plot shows the correlation of in vivo and in vitro half-lives of mRNA reporters. Half-life values were centered to medians. c, A boxplot shows positive correlation between GFP intensities and half-lives of mRNA reporters. All random sequences were divided into five groups based GFP intensity measured by flow cytometry. d, HEK293-Kb cells were transfected with DNA plasmids with or without optimal ATG codons (left) or representative hits from M and P fractions (right), followed by RT-qPCR at indicated time points (n = 3 biological replicates; t test). Error bars indicate SEM. ** P < 0.01; * P < 0.05. e, Representative flow cytometry scatterplots of HEK 293-Kb cells transfected with mRNA reporters capped with ApppG with sequence variants chosen from monosome (M1) or polysome (P1).
