Extended Data Fig. 2: Isolation of monomeric Aβ42 and antibodies by size exclusion chromatography.
From: Kinetic fingerprints differentiate the mechanisms of action of anti-Aβ antibodies
Isolation of the antibodies on a 1 × 30 cm Superdex 200 column (GE Healthcare) in 20 mM HEPES/NaOH, 140 mM NaCl, 1 mM CaCl2, pH 8.0 just prior to the kinetics experiment (first five panels). Isolation of recombinant Aβ1-42 on a 1 × 30 cm Superdex 75 column (GE Healthcare) in 20 mM HEPES/NaOH, 1 mM CaCl2, pH 8.0 just prior to the kinetics experiment (last panel). 140 mM NaCl was added from a 4.2 M NaCl stock after size exclusion to limit losses on the column. In each case, the fraction between the vertical red lines was collected.
