Extended Data Fig. 5: Dimer overlays reveal that calsequestrin structures can be classified into tightly packed or loosely packed dimers; related to Fig. 3.
From: The structure of a calsequestrin filament reveals mechanisms of familial arrhythmia
Dimers from published calsequestrin structures (lighter orange and green) are overlaid onto the tightly packed dimer from the present study (6OVW, darker orange and green). In each dimer pair, chain A is aligned to chain A to illustrate the relative displacement of chain B. The concentration of divalent cations used in the crystallization conditions is noted below. The overlays reveal two distinct conformational groupings. The more tightly packed conformation with inwardly rotated chains resembles the dimer in this study and appears to form at low pH or in the presence of neutralizing divalents.
