Extended Data Fig. 6: Tightly packed calsequestrin dimers consistently exhibit increased conformational disorder in domain I; related to Fig. 3.
From: The structure of a calsequestrin filament reveals mechanisms of familial arrhythmia
The top panel shows tightly packed calsequestrin dimers (that is dimers of calsequestrin crystallized in low pH or with high concentration of multivalent cations). In these structures, solvent-exposed loops in domain I are consistently disordered. In PDB 6OVW, the disordered loop region is omitted due to the high level of disorder. This same region (boxed, residues 58-68) is highly disordered in similar structures. The bottom panel shows loosely packed calsequestrin dimers (that is dimers of calsequestrin crystallized at neutral pH with low or trace concentrations of multivalent cations). The resolution for each structure is indicated, and several structures of non-comparable resolution are excluded (2VAF, 5CRE, 5KN0).
