Extended Data Fig. 5: Components of in vitro translation reaction.
From: SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation
(a) Predicted secondary structure of the 5’ region of SARS-CoV-2 genomic RNA (based on21,40) with 5’ UTR depicted in blue, the protein encoding sequence in black and the transcriptional regulatory sequence (TRS) in orange. Stem loops (SL) are labeled. (b) 1% agarose gel electrophoresis of the in vitro transcribed RLuc and FL-RLuc reporters used for the in vitro translation assays. (c) Input samples of the in vitro translation assay described in Fig. 3b. 2 μL of dialyzed Nsp1 protein samples at a concentration of 17.9 μM were analyzed on a 4–12% SDS-PAGE and stained by Imperial protein staining (Thermo Fisher Scientific).
