Extended Data Fig. 2: Data processing of the in vitro reconstituted 40S-Nsp1 cryo-EM dataset. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Data processing of the in vitro reconstituted 40S-Nsp1 cryo-EM dataset.

From: SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation

Extended Data Fig. 2: Data processing of the in vitro reconstituted 40S-Nsp1 cryo-EM dataset.

Scheme of the processing steps performed for the sample of the in vitro binding experiment. The local resolution estimate is plotted on the final volumes as heat map, together with additional slices through the volumes. The half map vs. half map FSC curves are plotted for the overall refinement (purple), the refinement focused on the body (blue), on the head (cyan), as well as for the composite map (green). The map vs. model FSCs are plotted for the body (yellow) and the head (orange) in their respective focused maps, as well as for the full 40S in the composite map (red).

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