Extended Data Fig. 6: Analysis of potential binding of PAR by HIRA.
From: Regulation of ALT-associated homology-directed repair by polyADP-ribosylation
a, GFP-PARP1, YFP-HIRA or GFP-FUS were transiently transfected in U2OS cells. Immunoprecipitated and blotted GFP-fusion proteins were incubated with biotinylated PAR and detected using an anti-PAR (10H) antibody. GFP fusion proteins were detected with HRP conjugated GFP antibody. b, Western blot showing depletion of endogenous HIRA and complementation with the indicated HIRA constructs in U2OS cells. Uncropped blot images for panel a,b are shown in Supplementary Fig. 1.
