Extended Data Fig. 7: Superposition of USP1-FANCD2 with USP12 and USP46.
From: Structural basis of FANCD2 deubiquitination by USP1−UAF1
The BL1 loop of USP1 (top; cryo-EM model) has an extension, compared to USP12 (middle; PDB 5L8W)24 and USP46 (bottom; PDB 5CVO)25, that is weakly visible in the cryo-EM map (locally filtered map contoured at a threshold of 0.2; see also Extended Data Figs. 1g and 6c). The BL2 loop of USP1 has an isoleucine (I587) that inserts into a hydrophobic pocket in FANCD2, whereas in USP12 and USP46 this residue is a serine (S311 and S307, respectively). FANCD2 is represented as a surface, with the other chains as ribbons.
