Extended Data Fig. 2: Cryo-EM data processing.
From: Structural basis of FANCD2 deubiquitination by USP1−UAF1
a, Gel filtration profile of the assembled USP1C90S-UAF1-FANCI-FANCD2Ub complex and associated SDS-PAGE and Coomassie staining. This experiment was performed once. The uncropped gel is provided as Source Data. b, Example micrograph from the dataset of 4591 after motion correction, CTF estimation, and manual curation. c, Example class averages. d, Unmasked, masked, and corrected Fourier Shell Correlation curves. e, Locally filtered map colored by local resolution. Batlow colouring was used. f, FSCs between the model and globally sharpened map g, Viewing direction distribution. h, Examples of well resolved regions from each protein subunit. The globally sharpened map within 2.5 Å of modelled atoms is shown and contoured at a threshold of 0.3.
