Extended Data Fig. 7: Catalytically active methyltransferase Dnmt3c is not expressed in DKO0 ESCs.
From: Dnmt1 has de novo activity targeted to transposable elements
a, RT-qPCR of Dnmt3c normalized to Rrm2. Lack of expression of Dnmt3c in WT ESC, TKOL, and DKO0. Levels of expression present in ESC were similar to the mouse embryonic fibroblast (MEF, negative control); mouse testes serve as a positive control (n = 3, testes collected from three individual mice). Bars denote the mean and the error bars denote mean plus standard deviation. WGBS and ChIP-seq (top: WGBS, middle: H3K4me3, bottom; H3K36me3) at the Dnmt3b and Dnmt3c locus in the DKO0 state. Lack of H3K4me3 at the promoter and H3K36me3 across the gene body of Dnmt3c suggests no detectable transcription (WGBS n = 1, H3K4me3 n = 2, H3K36me3 n = 1). b, Re-analysis of splice sites of the RNA-seq from Wang et al.19, showing deletion associated read-through of the Dnmt3b promoter into the Dnmt3c gene, resulting in high read count levels for catalytic Dnmt3c isoforms.
