Extended Data Fig. 3: Workflow for cryo-EM data processing of S-UK:ACE2.
From: Effect of SARS-CoV-2 B.1.1.7 mutations on spike protein structure and function
Overview of the cryo-EM data processing of SARS-CoV-2 S-UK in complex with the ACE2 ectodomain. The same processing procedure as described in Extended Data Fig. 1 was used to yield five distinct structures based on the 3DVA. Each structure is shown in semitransparent surface and fit to the most populated structure (solid pink structure) from the top view to highlight the domain movements of the individual ACE2 ectodomains. To obtain a high-resolution structure of ACE2 in complex with the S-UK, a mask was generated (semitransparent volume in the bottom gray EM map) for the local refinement, which yielded a 3.5-Å cryo-EM map as shown on the bottom right panel. ACE2 and RBD are colored in magenta and blue, respectively. A representative micrograph as the source data is available online (Source Data Fig. 2).
