Extended Data Fig. 3: Comparison of the inactive β1-AR receptor with the activated β1-AR in complex with Gi. | Nature Structural & Molecular Biology

Extended Data Fig. 3: Comparison of the inactive β1-AR receptor with the activated β1-AR in complex with Gi.

From: Structural basis and mechanism of activation of two different families of G proteins by the same GPCR

Extended Data Fig. 3

(a-c) Side (left), extracellular (top right) and cytoplasmic (bottom right) views of the inactive β1-AR (grey)(PDB code 4GPO) compared to the activated β1-AR in complex with Gi. (d) Structural comparison of the Gi α5-helix binding region to show the major conformational changes of β1-AR. (e) The ionic lock between Arg139 and Glu285 is broken in the active state. (f) Tyr343 packs against Leu132 and Ile135 in the active state. (g) Cryo-EM density map of the Gi α5-helix binding region (contoured at 1.2 σ level).

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