Extended Data Fig. 3: AlphaFold2 model phasing of the GP2 branch P1 X-ray data. | Nature Structural & Molecular Biology

Extended Data Fig. 3: AlphaFold2 model phasing of the GP2 branch P1 X-ray data.

From: Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH

Extended Data Fig. 3

a, Superposition of five AlphaFold2 models of the GP2 N-terminal branch indicates the presence of three distinct units, with a central hairpin-like region (residues D45-F60; orange box) separating an N-terminal low-confidence region (residues V29-L44; red box) from a C-terminal globular domain (residues D61-S181; green box). An ensemble corresponding to the latter was used as search model for MR. b-c, Electron density for an Endo H cleavage-derived N-acetylglucosamine residue attached to N122 (b) and the hairpin region (c), two GP2 elements not included in the MR search ensemble. Fourier maps at different stages of the structure determination process are shown, contoured at the indicated levels.

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