Extended Data Fig. 6: Assembly of the composite map of full-length UMOD. | Nature Structural & Molecular Biology

Extended Data Fig. 6: Assembly of the composite map of full-length UMOD.

From: Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH

Extended Data Fig. 6

Multi-body refinement of the UMOD filament core plus D10C domain (left path) and the whole UMOD branch (right path) were performed separately. Helical symmetry was applied to the filament core plus D10C, after the best homogenous filamentous segments were selected based on 2D classes. Meanwhile, the particles with the better contrast, more extended branch features were independently selected, locally 3D classified and refined, without helical symmetry. The final composite map was assembled by merging copies of the branch with the filament core plus D10C.

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