Extended Data Fig. 1: Purification and analysis of Rad24-RFC:9-1-1.
From: Mechanisms of loading and release of the 9-1-1 checkpoint clamp
a, Representative Coomassie-stained SDS-PAGE analysis of purified S. cerevisiae Rad24-RFC, 9-1-1 and RPA. The * corresponds to truncated form of Rad24. b, ATPase activity of Rad24-RFC in the presence of 9-1-1 and DNA (black circles), Rad24-RFC in the presence of DNA (teal triangles), Rad24-RFC in the presence of 9-1-1 (purple triangles) and 9-1-1 and DNA (pink squares). Experiments are shown in triplicate. c, Schematic for the assembly of Rad24-RFC:9-1-1:DNA in the presence of ATPγS. d, Representative silver-stained SDS-PAGE analysis of Rad24-RFC:9-1-1 fractions following glycerol gradient (10-35%) centrifugation. Fractions pooled for Cryo-EM analysis are denoted with *. e, Representative Coomassie-stained SDS-PAGE analysis of purified S. cerevisiae Rad24-RFC:9-1-1:DNA. Fractionation experiments shown in panels d and e have been repeated at least three times.
