Extended Data Fig. 2: Validation of Rad24-RFC:9-1-1 structures. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Validation of Rad24-RFC:9-1-1 structures.

From: Mechanisms of loading and release of the 9-1-1 checkpoint clamp

Extended Data Fig. 2

a, Representative cryo-EM image of vitrified Rad24-RFC:9-1-1:DNA. b, Representative two-dimensional averages of Rad24-RFC:9-1-1. c, Plot of Fourier shell correlations between two independent open state half-maps (blue), two independent closed state half-maps (red), two independent Mec3 focus half-maps (brown), the open state map and open state atomic model (cyan), and the closed state map and closed state atomic model (magenta). d-f Cryo-EM density maps of open (d), Mec3-focused refined (e) and closed (f) maps colored by local resolution in Å. Region included in the mask for the Mec3 focused refinement (e) is denoted by the dashed line in d.

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