Extended Data Fig. 5: ÎĽs-ms dynamics in Ca2+-ADP-actin.
From: Bound nucleotide can control the dynamic architecture of monomeric actin
a, Multiple quantum relaxation dispersion (ΔR2), difference in 1H/13C R2eff between the lowest and highest CPMG field strengths, recorded at 800 MHz of Ile δ1-methyl resonances of 1H/13C- Ile δ1-methyl-labeled Ca2+-ADP-actin. Error bars in ΔR2 represent the SD based on noise levels in NMR spectra and were calculated using standard error propagation methods b, Absolute value of the difference between ground and excited state 13C and 1H chemical shifts |Δω| for Ile δ1-methyl resonances of Ca2+-ADP-actin determined from global fitting of the MQ and 1H SQ CPMG data. c, Thermodynamic (Pb) and kinetic parameters (kex) obtained from two-state global fitting. Errors in |Δω|, Pb, and kex were determined from the covariance matrix of the global fits.
