Extended Data Fig. 6: Comparison of dynamics in ATP- and ADP-bound G-actin.
From: Bound nucleotide can control the dynamic architecture of monomeric actin
a, Comparison of MQ ΔR2 recorded at 800 MHz of Ile δ1-methyl resonances of 1H/13C- Ile δ1-methyl-labeled ATP- (red) and ADP-bound (green) actin. Only residues I64, I71, I75 and I85 showed ΔR2 ≥ 2 Hz in ADP-actin, whereas many additional residues showed ΔR2 ≥ 2 Hz in ATP-actin. Error bars in ΔR2 represent the SD, based on noise levels in NMR spectra and were calculated using standard error propagation methods b, Although, residues showing ΔR2 ≥ 2 Hz are mainly confined to SD1 and SD2, in ADP-actin, the magnitude of ΔR2 was higher due to a greater population of the excited state. The error bars represent the SD (standard deviation), calculated from the two and three replicated data points for 600 and 800 MHz data, respectively.c, Hydrolysis of ATP (red) into ADP (yellow) triggers a series of rearrangements from which multiple hydrogen bonds (shown as dotted line) are broken.
