Fig. 2: Chemical shift changes due to the nucleotide switch propagate throughout G-actin.
From: Bound nucleotide can control the dynamic architecture of monomeric actin
a, Overlaid 1H/13C methyl TROSY NMR spectra of Ca2+-actin in the ATP- (red) and ADP-bound (black) forms. b,c, Chemical shift differences (Δ = [(δ1Η)2 + (0.25 × δ13C)2]1/2) between the ATP- and ADP-bound forms of actin. Residues showing Δ > 0.04 ppm (black line) are colored red (b), and are labeled in a and shown as red spheres in the wire representation of G-actin (PDB 2HF4) (c). SDs are colored as in Fig. 1c. d,e, Comparison of AP mutant G-actin crystal structures in the ATP- (PDB 2HF4; cyan) and ADP-bound (PDB 2HF3; wheat) forms32 (d). The structures in the two states are nearly identical except in the sensor loop region with an overall backbone r.m.s.d. of 0.23 Å (e). Panels c–e show stereo views.
