Fig. 4: G-actin has a different dynamic architecture in its ATP- and ADP-bound forms.
From: Bound nucleotide can control the dynamic architecture of monomeric actin
a–c, Isoleucine residues with MQ or 1H SQ relaxation dispersion > 2 Hz mapped onto the G-actin structure in the ATP- (left panel, red balls; PDB 2HF4) and ADP-bound (right panel, green balls; PDB 2HF3) forms (a). Black balls, overlapped residues (I136, I309, I369) in NMR spectra. The SDs are colored as in Fig. 1c. Panels a and b were generated using Chimera. 3D (b) and 2D (c) depictions of the NBS of ATP- and ADP-bound actin (left and right images, respectively). Hydrogen bonds are indicated by dashed lines. In ATP-actin, the two halves of the structure are bridged through additional hydrogen bonds between the terminal γ-phosphate and residues in SD3 that are absent in ADP-actin (circled in red). Panel c was generated using LigPlot+.
