Extended Data Fig. 4: Cryo-EM analysis of the IR-insulin site-2 mutant (LeuA13R) complex.
From: Synergistic activation of the insulin receptor via two distinct sites
a. Representative electron micrograph and 2D class averages of the IR-insulin site-2 mutant (LeuA13R) complex. Scale bar: 200 Å. This experiment was repeated for 3,783 times independently with similar results. b. Unsharpened cryo-EM map colored by local resolution. c. The gold-standard Fourier Shell Correlation (FSC) curve for the cryo-EM map shown in Fig. 3a. d. Flowchart of cryo-EM data processing.
