Extended Data Fig. 2: CryoEM analysis of CENP-N mediated stacks of CENP-A mono-nucleosomes reconstituted with palindromic α-satellite DNA. | Nature Structural & Molecular Biology

Extended Data Fig. 2: CryoEM analysis of CENP-N mediated stacks of CENP-A mono-nucleosomes reconstituted with palindromic α-satellite DNA.

From: CENP-N promotes the compaction of centromeric chromatin

Extended Data Fig. 2

a) Flow-chart of the analysis of di-nucleosomes in the stacks. b) 2D classification of di-nucleosomes with CENP-N. c) Local resolution of the 3D map for stacked CENP-A nucleosomes in complex with CENP-N. d) 3DFSC analysis of the reconstructed 3D map. The plot reveals significant orientation bias in the map reconstruction. Therefore, the estimated global resolution of 3.54 Å does not represent the overall map quality. In some orientations, the resolution is closer to 10 Å.

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