Extended Data Fig. 2: Protein identification strategies. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Protein identification strategies.

From: Ciliary central apparatus structure reveals mechanisms of microtubule patterning

Extended Data Fig. 2

a, A flow diagram showing the strategies used to identify proteins in the C1 and C2 maps. If no matches were found in the lists of candidate proteins from mass spectrometry analysis11,12, the search was expanded to include the entire Chlamydomonas proteome. b, An example showing how DeepTracer70 was used to identify FAP213. c, Examples of density for proteins that are resolved at the side chain level (FAP388 and FAP147) and those that have regions that are only resolved at the domain level (FAP101, Hydin and PF16). Extended Data Table 1 lists the built regions of each identified protein and whether there is side chain information.

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