Fig. 2: Cohesin acetylation restricts loop length in a WAPL-independent manner.
From: The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
a, Hi-C contact matrices for G1 cells of the indicated genotypes. A locus at chromosome 5 is shown at 10-kb resolution. Matrices were normalized to 100 million contacts per sample. b, Immunoblot analysis of the indicated genotypes. This experiment was performed twice, with similar results. c, APA for extended loops in the indicated genotypes (top); differential APA plots for extended loops compared with ∆WAPL (∆W) cells (bottom). ∆ESCO1/∆WAPL (∆1/∆W) cells show an increase in extended loops in comparison with ∆WAPL cells. d, Aggregate stripe analysis to quantify signal enrichment emanating from CTCF sites at 100-kb resolution. Both ∆ESCO1 and ∆WAPL cells display longer stripes in comparison with wild-type cells, although stripes are extended even further in ∆ESCO1/∆WAPL cells.
