Fig. 4: Cohesin acetylation controls loop length through PDS5A.
From: The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
a, Immunoblot analysis of the indicated genotypes. This experiment was performed three times, with similar results. b, Hi-C contact matrices for G1 cells of the indicated genotypes. A locus at chromosome 5 is shown at 10-kb resolution. Matrices were normalized to 100 million contacts per sample. c, APA for primary loops (top). The bottom row displays differential APA plots for primary loops in ∆HDAC8 (∆8) cells, ∆PDS5A (∆5 A) cells and ∆HDAC8/∆PDS5A (∆8/∆5A) cells in comparison with wild-type (WT) cells. ∆PDS5A and ∆HDAC8/∆PDS5A cells show a decrease in primary loops. d, APA for extended loops (top). The bottom row displays differential APA plots for extended loops compared with wild type. ∆HDAC8 cells show a decrease in extended loops; this defect was rescued following deletion of PDS5A. ∆PDS5A and ∆HDAC8/∆PDS5A cells both show an increase in extended loops. e, Aggregate stripe analysis to quantify signal enrichment emanating from CTCF sites at 100-kb resolution. Deletion of PDS5A in ∆HDAC8 cells rescued the shorter interactions found in ∆HDAC8 cells.
