Extended Data Fig. 2: Hi-C replicates in G1 cells.
From: The cohesin acetylation cycle controls chromatin loop length through a PDS5A brake mechanism
(a) Aggregate peak analysis (APA) reveals that the extended loop phenotype is also observed in a replicate Hi-C experiment. ∆ESCO1/∆WAPL cells show an increase in extended loops in comparison to ∆WAPL cells. (b) The extended stripe phenotypes for ∆WAPL and ∆ESCO1/∆WAPL cells are also observed in a replicate Hi-C experiment. The aggregate stripe analysis quantifies the signal enrichment emanating from CTCF sites at 100-kb resolution. (c) APA plots show that the extended loop phenotypes are also observed in a replicate Hi-C experiment. For ∆PDS5A (∆5 A) cells we used an independent clone, for ∆HDAC8/∆PDS5A (∆8/∆5A) cells we used the same clone. (d) The extended stripe phenotypes for ∆PDS5A and ∆HDAC8/∆PDS5A cells are also observed in a replicate Hi-C experiment in the clones described in (c). The aggregate stripe analysis quantifies the signal enrichment emanating from CTCF sites at 100-kb resolution. (e) APA in an independent ∆PDS5B clone confirms that PDS5B does not regulate the formation of extended loops. (f) Hi-C analysis in an independent ∆PDS5B clone confirms that PDS5B does not regulate the length of architectural stripes.
