Extended Data Fig. 1: Characterization of cell lines by Sir-DNA dye incorporation.
A) Left panel, wildtype, Per DKO, Per TKO cells and mPer2 rescue fibroblasts were treated with DMSO or 1mM of siR-DNA live cell Fluorogenic Labelling Probe (SpirochromeSiR-DNA) and analyzed by flow cytometry (representative of N=3 experiments). Right panel, Results of three independent experiments are represented as mean±SD of the geometric mean. A one-tailed t-test was performed. B) Total genomic DNA from Per TKO cells, mPer2 rescue cell lines, wildtype fibroblasts and Per DKO cells was extracted and quantified. Results are expressed as mean±SD of N=3 independent experiments. A one-tailed t-test was performed. C) Sequential salt extraction (80-600mM NaCl) of wildtype and Per TKO nuclei were performed. Extracts as well as the final pellet were blotted for H2A.Z and H3. Results are representative of 4 experiments.
