Extended Data Fig. 7: SPA data processing of apo GiGet3. | Nature Structural & Molecular Biology

Extended Data Fig. 7: SPA data processing of apo GiGet3.

From: Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3

Extended Data Fig. 7

A) An aligned and dose weighted micrograph from the data collection (7,607 micrographs) with apo GiGet3 sample particles selected with green circles. Scale bar represents 50nm. B) 2D class averages of particles used for the reconstruction. C) Processing of data through cryoSPARCv3.2.0 and RELION 3.1.2. D) Two views of the angular distribution of particles. Particle concentration is displayed by color and length (blue to red). Density filtered to 7 sigma for various helices that are shown as sticks: E) H7 (cyan) & H9 (magenta) and F) H10 (magenta) & H11 (cyan).

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