Extended Data Fig. 3: Alignment of Get4 homologs.

A) (Left) AlphaFold2 structural prediction of the identified GiGet4 colored based on confidence from blue (most confident) to orange (least confident). Experimentally determined structure of the yeast Get4 (center)14 and human Get4 (PDBID: 6AU8) (right)93 are colored from N- to C-terminus using the viridis color scheme (purple to yellow). B) Alignment of Get4 homologs including: G. intestinalis, H. sapiens, S. cerevisiae, A. queenslandica, S. pombe, N. crassa, A. fumigatus, and P. falciparum. Conserved residues that were demonstrated to play a role in Get3 binding are highlighted with asterisks above. Residues are colored as in Extended Data Fig. 122.