Extended Data Fig. 4: Identification of a Get2 homolog in G. intestinalis. | Nature Structural & Molecular Biology

Extended Data Fig. 4: Identification of a Get2 homolog in G. intestinalis.

From: Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3

Extended Data Fig. 4

A) An alignment of the identified Get2 from G. intestinalis with CAML from H. sapiens and Get2 from S. cerevisiae. The three predicted TMDs are shown with red cylinders above the alignment and the conserved N-terminal helices that tether to Get3 are shown as blue cylinders. The two conserved residues involved in Get3 binding are marked by asterisks below the alignment. Residues are colored using the ClustalX color scheme. Representative images from three experiments for (B) detection of GiGet2 using Stimulated Emission Depletion (STED) microscopy images of G. intestinalis trophozites. Get2 with a C-terminal V5 tag was detected by an anti-V5 antibody (yellow) and the ER membrane was labeled by an anti-PDI2 antibody (magenta). Images are merged in the third panel. (C) The ER localization of GiGet2 differs from the cellular localization of GiGet3. GiGet3 with a N-terminal HA-tag was labeled by an anti-HA antibody (yellow) and GiGet2 is labeled by the same method in (A) (magenta). Images are merged in the third panel. Scale bars represent 5µm.

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