Extended Data Fig. 2: Schematics of data processing.
From: In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes
a, A schematic of the processing workflow of 96 nm-repeating units of doublets to achieve the consensus average combining data from all nine doublets. An example of remapping of the centers of the final subvolumes in three dimensions is shown. b, A schematic of the processing workflow of 32 nm-repeating units of central complex to achieve the subvolume average. Recentering of subvolumes of the central pair complex (every 32 nm) on the doublet 1 is shown. Remapping of these subvolumes helps to trace the trajectory of doublet 1 in three dimensions. The centers of these doublet 1 subvolumes are shown in the same tomogram as in a. c, A schematic of the workflow of multi-body refinement.
