Extended Data Fig. 2: Bifurcation in unique sequences used to find E-value cutoff.

The number of sequences in multiple sequence alignments constructed using cGAS (orange) or MiD-51 (blue) input sequences, as well as the number of unique sequences found only in the cGAS or MiD51 alignment but not both (grey), and the total number of sequences (black) plotted as a function of the E-value cutoff used to generate the alignment. The ratio of unique sequences to total sequences (green) is plotted on the secondary y axis.