Extended Data Fig. 8: In vitro nucleophile reactivity screening.
From: Genetic code expansion reveals aminoacylated lysine ubiquitination mediated by UBE2W
All Ub-transferred E2s were purified and the reactivity of E2~Ubs towards buffer (-), K, PraK and AsnK was determined. Reactions were quenched by a non-reducing loading buffer. Reactivity of E2~Ub with lysine or aminoacylated lysines is indicated by the decrease of E2~Ub conjugates and the increase of free E2 and free Ub. E2~Ub and free E2 are indicated by the red and black triangles, respectively. Free Ub is highlighted by the dashed boxes. The experiment in the figure was repeated twice with similar results. Source data are provided as a Source Data file.
