Extended Data Fig. 3: The representative spectra of K-XUb modification.
From: Genetic code expansion reveals aminoacylated lysine ubiquitination mediated by UBE2W
a, A representative spectrum of K-MUb on K98 of PSB3 protein. Inset: enlarged signal for the characteristic fragment (GGM). b, A representative spectrum of K-oxMUb on K1214 of TOP2B protein. Inset: enlarged signal for the characteristic fragment (GGoxM, oxM refers to the oxidized form of Met). c, The spectrum of synthesized peptide with a branching GGM modification on lysine. Inset: enlarged signal for the characteristic fragment (GGM). d, The spectrum of synthesized peptide without a branching GGM modification on lysine. Inset: enlarged signal for the non-existence of characteristic fragment (GGM). e, A representative spectrum of K-XleUb on K338 of TBA1A protein in UbiSite enrichment. Inset: enlarged signal for the characteristic fragment (GGXle, Xle refers to the Leu or Ile). f, A spectrum of K-NUb on K49 of 14-3-3Z protein in UbiSite enrichment. Inset: enlarged signal for the characteristic fragment (GGN).
