Extended Data Fig. 5: Cryo-EM data processing workflow for nanodisc-stabilized CgtWT with Mg2+, ADP, sodium orthovanadate and CβG (CgtVAN).
From: Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella
a, Representative motion-corrected cryo-EM micrograph (scale bar is 50 nm). b, representative 2D class averages. c, Angular distribution of the cryo-EM particles included in the final reconstruction. d, Single-particle cryo-EM data processing scheme using cryoSparc 3.1. e, Final 3D reconstruction colored according to local resolution. f, Fourier shell correlation curves for the final 3D reconstruction. g, TM helices of the CgtVAN model overlapping with the corresponding experimental density. Contour levels are 0.5. Cryo-EM data was obtained in a single data collection session (n = 1).
