Extended Data Fig. 9: Cgt substrate translocation.
From: Mechanism of cyclic β-glucan export by ABC transporter Cgt of Brucella
a, Side view of the central cavity of the CgtSUB (left) and CgtDET (right) models, indicating the CβG binding pocket residues (purple) and the free space in the central cavity (blue shading). Residues pointing towards the center of the inner cavity in both models that were targeted by Trp mutagenesis are shown in yellow. b, View of the inner part of the central cavity from the periplasmic space in CgtSUB (top) and CgtDET (bottom) models. Residues mutated to Trp are shown in yellow. c, d, In-vivo Cgt activity assay indicating the ability of different Trp mutants of Cgt to restore growth of Atu Δcgt under osmotic stress. Both growth curves (c) and a single 20 h time point (d) are shown. Growth curves represent mean values of 3 independent experiments (n = 3). Dots represent mean values of independent experiments (n = 3). Error bars represent standard deviation. Data for graphs c-d are available as source data.
