Fig. 5: Recursive design of higher-order assemblies.
From: De novo design of obligate ABC-type heterotrimeric proteins
a, SAXS profiles indicate a good quality of fit (χ) between the design model (line) and experimental scattering data (black dots) for four A2B2 tetramers: three type I tetramers (C2-DHT01-01, C2-DHT01-02 and C2-DHT01-03) and one type II tetramer (C2-DHT01-04). In panels b-e, design models are shown with different chains in different colors, superimposed on nsEM reconstructed 3D maps, with 2D class averages on the right. b, a type I A2B2 heterotetramer (C2-DHT01-03; top) and a type II A2B2 heterotetramer (C2-DHT01-04; bottom). c, Characterization of two A3B3C3 cyclic designs: C3-DHT03-01 (top) and C3-DHT03-02 (bottom). d, Characterization of two A4B4C4 cyclic designs: C4-DHT03-01 (top) and C4-DHT03-02 (bottom). e, Fusion accessibility of the a third chain enables further design opportunities. Versions of C3-DHT03-01 and C4-DHT03-01 with DHR82 fused to chain C of DHT03.
