Extended Data Fig. 5: SWI/SNF complex interacts with AP-1 complex and colocalizes on genome. | Nature Structural & Molecular Biology

Extended Data Fig. 5: SWI/SNF complex interacts with AP-1 complex and colocalizes on genome.

From: Cooperation of chromatin remodeling SWI/SNF complex and pioneer factor AP-1 shapes 3D enhancer landscapes

Extended Data Fig. 5

a. Western blot of G401 cells at Day 0 and 3 of SMARCB1 addback Immuno-Precipitated with AP-1 family proteins. b. Venn diagram comparison of CUT&RUN peak numbers of AP-1 subunits pJUN, JUND, and JUNB with ARID1A, PBRM1, and BRD9 without SMARCB1 (Day 0) and SMARCB1 re-expression (Day 1 and Day 3). c. Heat maps of SWI/SNF subunits ARID1A, PBRM1, BRD9, SMARCC1, and AP-1 subunits pJUN and JUNB aligned to all JUND peaks in G401 cells. TSS-proximal is defined ±2 Kb of TSS, TSS-distal is all regions outside 2 Kb of TSS. d. Heat maps of SWI/SNF subunits ARID1A, PBRM1, BRD9, SMARCC1, and AP-1 subunits pJUN and JUNB aligned to all JUNB peaks in G401 cells. TSS-proximal is defined ±2 Kb of TSS, TSS-distal is all regions outside 2 Kb of TSS. e. Average profile of SMARCC1 and ARID1A CUT&RUN signal before and after SMARCB1 addback following AP-1 depletion compared to a non-targeting control.

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