Extended Data Fig. 1: Massively parallel mutagenesis of NOG2.
From: A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome
A. Scheme for high throughput mutagenesis and analysis of functional variants of NOG2. B. Complete fitness landscape for variants in the core GTP-binding motifs of NOG2 (G4, G1, G2, G3; amino acids 260-369). Yellow squares indicate the WT amino acid identity. C. Distributions of observed fitness scores (Φ) for nonsense (red), missense (gray) and synonymous (green) mutations. D. Correlation between per-residue median fitness score and ConSURF 41 score (evolutionary conservation: scores 1-3, low; scores 4-6, medium; scores 7-9, high conservation). The statistical analysis used was performed in TileSeq and described in Weile et al. (ref. 8).
