Extended Data Fig. 3: Schematic of primer extension probing 2′-O-methylation at G2922. | Nature Structural & Molecular Biology

Extended Data Fig. 3: Schematic of primer extension probing 2′-O-methylation at G2922.

From: A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome

Extended Data Fig. 3: Schematic of primer extension probing 2′-O-methylation at G2922.

A. RNase T1 cleaves 3′ to guanosines in single-stranded RNA. However, 2′-O-methylated guanosine is resistant to cleavage. Guanosines base-paired with the reverse transcription primer are also protected. After cleavage with RNase T1, reverse transcription will generate a 30 nt product if G2922 is sensitive to cleavage (that is not methylated), or a 34 nt product if G2922 is protected from cleavage (that is methylated).

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