Extended Data Fig. 5: Details of cryo-EM data collection and processing.
From: A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome
A. Raw micrograph of Nog2-3xFLAG particles immunopurified from the pGAL:SPB1 strain AJY4425 expressing spb1- D52A. Representative of 25,956 micrographs. Scale bar = 100 nm. B. Cryo-EM data processing scheme by which the Nog2-3xFLAG structure was obtained.
