Table 1 Cryo-EM data collection, refinement and validation statistics
From: A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome
Data collection and processing | |
Magnification | ×29,000 |
Voltage (kV) | 300 |
Electron exposure (e– Å−2) | 70 |
Defocus range (μm) | −1.5 to −2.5 |
Pixel size (Å) | 0.81 |
Symmetry imposed | C1 (that is, none) |
Initial particle images (no.) | 403,566 |
Final particle images (no.) | 15,954 |
Map resolution (Å) | 2.9 |
FSC threshold | 0.143 |
Map resolution range (Å) | 2–10 |
Refinement | |
Initial model used (PDB accession no.) | |
Model resolution (Å) | 2.9 |
FSC threshold | 0.5 |
Model resolution range (Å) | Not applicable |
Map sharpening B factor (Å2) | 19.0 |
Model composition | |
Nonhydrogen atoms | 140,993 |
Protein residues | 8,739 |
Nucleotides | 3,338 |
Ligands | Mg2+: 1 |
B factors (Å2) | |
Protein | 38.9 |
Ligand | 62.99 |
R.m.s. deviations | |
Bond lengths (Å) | 0.010 |
Bond angles (°) | 0.880 |
Validation | |
MolProbity score | 1.88 |
Clashscore | 11.12 |
Poor rotamers (%) | 0.01 |
Ramachandran plot | |
Favored (%) | 95.37 |
Allowed (%) | 4.62 |
Disallowed (%) | 0.01 |
