Fig. 6: Analysis of lipid bilayer composition at membrane–protein contact sites and between contact sites from simulation and experiment.
From: Brominated lipid probes expose structural asymmetries in constricted membranes
a,b, Radial profile of cryo-EM density (a) at CHMP1B contact sites and (b) between contact sites for the unbrominated and SDPC-Br samples. See Extended Data Fig. 4c,d for all profiles. c, The molar composition of each leaflet at membrane–protein contact sites (top) and at the gaps (bottom). Uncertainty was calculated as the standard deviation from independent calculations from each of the two half maps. d, The corresponding compositions for these two sections from CG-MD are shown for the contact sites (top) and at the gaps (bottom) from simulations using the enriched lipid composition. Uncertainty was calculated as the standard deviation between three replicates of CG-MD trajectories. e, Cartoon model of bilayer structure and composition at CHMP1B contact sites. Data for graphs in a and b are available as source data.
